Journal: Journal of Clinical Laboratory Analysis

Article Title: High‐dimensional single‐cell proteomics analysis reveals the landscape of immune cells and stem‐like cells in renal tumors

doi: 10.1002/jcla.23155

Figure Lengend Snippet: Purified antibodies about the immune cell‐centric panel

Article Snippet: CD161 , 164Dy , HP‐3G10 , Fluidigm , 3164009B.

Techniques: Purification

Journal: iScience

Article Title: IL-23 tunes inflammatory functions of human mucosal-associated invariant T cells

doi: 10.1016/j.isci.2025.111898

Figure Lengend Snippet: IL-23 enhances production of cytotoxic molecules and IL-10 by MAIT cells (A) Percentage of IL-23R positive cells in different immune cell populations was assessed by flow cytometry on freshly isolated PBMCs ( n = 11). (B and C) PBMC were activated with Tet for 6 days or left unstimulated (black). Sorted MAIT cells (CD3 + CD161 + Va7.2 + ) were re-stimulated with Tet in the absence (gray) or presence of IL-23 (red) or IL-12 (blue) for 24 h. Cytokine secretion in the supernatants was measured using Luminex technology ( n = 9). (B) Heatmap of measured cytokines. Dendograms on the top and left sides correspond to hierarchical clustering. (C) Boxplots (lines correspond to the first quartile, the median and the third quartile) representing the concentration of the indicated cytokines (adjusted p values, paired Wilcoxon test, Benjamini-Hochberg correction). (D) Single cell secretome of MAIT cells activated for 6 days with Tet in the presence or absence of IL-23 or IL-12 was assessed using Isolight technology. The heatmap represents the single-cell co-secretion patterns and their frequencies ( n = 4). The values for granzyme B are ∼15% for the Tet+IL-23 condition, and ∼17% for Tet+IL-12. See also Figure S1 and Tables S1 and .

Article Snippet: CD161 BV421 (Clone HP-3G10) , Sony Biotechnology , Cat#2299570.

Techniques: Flow Cytometry, Isolation, Luminex, Concentration Assay

Journal: iScience

Article Title: IL-23 tunes inflammatory functions of human mucosal-associated invariant T cells

doi: 10.1016/j.isci.2025.111898

Figure Lengend Snippet: Genes and pathways regulated by IL-23 or IL-12 in MAIT cells PBMCs were activated with Tet in the presence or absence of IL-23 or IL-12 for 6 days, CD3 + CD161 + Va7.2 + MAIT cells were sorted, and RNA-sequencing was performed. (n = 5–8) (A) Scatterplot of changes in gene expression (log2-fold change, x axis: Tet+IL-23 vs. Tet, y axis: Tet+IL-12 vs. Tet). Each dot corresponds to a differentially expressed gene (red: Tet+IL-23 vs. Tet, blue: Tet+IL-12 vs. Tet, purple: Tet+IL-23 vs. Tet and Tet+IL-12 vs. Tet). Gray shading indicates the 2D density plot of all the delta log-fold change. (B) Euler diagram representing the differentially express genes in the Tet+IL-23 vs. Tet, and in the Tet+IL-12 vs. Tet stimulation conditions. (C and D) Over-representation analysis was performed on the genes regulated by (C) IL-23 or (D) IL-12 using the GLAD4U database. Bubble plots of the top 20 associated pathways. Orange asterisks indicate pathways associated with chronic inflammatory and autoimmune diseases pathways. Blue asterisks indicate pathways associated with infectious diseases. See also Figure S7 and Tables S5 , , , and .

Article Snippet: CD161 BV421 (Clone HP-3G10) , Sony Biotechnology , Cat#2299570.

Techniques: RNA Sequencing, Gene Expression

Journal: iScience

Article Title: IL-23 tunes inflammatory functions of human mucosal-associated invariant T cells

doi: 10.1016/j.isci.2025.111898

Figure Lengend Snippet: IL-23 regulates MHC class II and AP-1 transcription factor genes in MAIT cells PBMCs were activated with Tet in the presence or absence of IL-23 or IL-12 for 6 days, CD3 + CD161 + Va7.2 + MAIT cells were sorted, and RNA-sequencing was performed. (n = 5–8). (A) Heatmap of the expression of 65 genes associated with autoimmune pathways. Gray: Tet, red: Tet+IL-23, blue: Tet+IL-12. (B) Boxplots of the expression of HLA genes. Differential gene expression analysis (limma, adjusted p values, TPM: transcripts per million). (C) PBMCs were left unstimulated or activated with Tet in the presence or absence of IL-23 or IL-12 for 6 days, then stained for HLA-II expression. Frequency of HLA-DR, HLA-DM, and HLA-DP positive cells in the MAIT population. ( n = 6, paired t test). (D) Boxplots of the expression of AP-1 transcription factor genes.

Article Snippet: CD161 BV421 (Clone HP-3G10) , Sony Biotechnology , Cat#2299570.

Techniques: RNA Sequencing, Expressing, Gene Expression, Staining

Journal: iScience

Article Title: IL-23 tunes inflammatory functions of human mucosal-associated invariant T cells

doi: 10.1016/j.isci.2025.111898

Figure Lengend Snippet: IL-23 signals in MAIT cells are mediated by the AP-1 transcription factors BATF, JUNB, and BACH2 (A) Genome-wide in silico footprinting analysis was computed on ATAC-seq data from MAIT cells activated with Tet ± IL-23. Volcano plot of the genome-wide transcription factor binding changes. Each dot represents one motif, empty dots indicate non-significant events. (B) Scatterplot displaying log2-fold change of gene expression against differential binding score for each of the transcription factors used for footprinting. In the case of heterodimers, the gene expression log2-fold change corresponds to the mean of the two genes. Gray dashed lines link the different TF binding prediction for TF binding to several motifs. (C–E) PBMC were activated with Tet for 6 days. At day 5 of culture, PBMC were transfected with fluorescently labeled Cas9 RNP targeting BATF (Orange) or non-targeting (Control, gray). At day 6, transfected MAIT cells (CD3 + CD161 + Va7.2 + ATTO550 + ) were sorted, cultured for 4 additional days and gene expression and cytokine secretion in the supernatant were assessed ( n = 3). (C) Schematic representation of the experimental design. (D) Boxplots representing gene expression (normalized RNA counts, nCounter technology) of BATF and HLA-DRA ( n = 3, p -values, paired t test). (E) Concentration of IL-10 measured in the supernatant using MSD technology ( n = 3). See also Table S11 .

Article Snippet: CD161 BV421 (Clone HP-3G10) , Sony Biotechnology , Cat#2299570.

Techniques: Genome Wide, In Silico, Footprinting, Binding Assay, Gene Expression, Transfection, Labeling, Control, Cell Culture, Concentration Assay

Journal: iScience

Article Title: IL-23 tunes inflammatory functions of human mucosal-associated invariant T cells

doi: 10.1016/j.isci.2025.111898

Figure Lengend Snippet:

Article Snippet: CD161 BV421 (Clone HP-3G10) , Sony Biotechnology , Cat#2299570.

Techniques: Control, Recombinant, CRISPR, Negative Control, Software